"Ich habe die Antikörper sowohl mittels Western Blot als auch Immunfluoreszenz getestet. In beiden Fällen sind die Resultate sehr gut. Beide Antikörper (…) erkennen nur das phosphorylierte Protein."

A customer's quote

Published work relying on immunoGlobe services (selection):

  • Wild et al. (2011) Science 333:228-33.
  • Günther et al. (2009) J. Mol. Med. 87:31-41.
  • Pagenstecher et al. (2009) Hum. Mol. Genetics 18: 911–918.
  • Fritsche et al. (2008) Nature Genetics 40: 892-896.
  • Agerer et al. (2003) J. Biol. Chem. 278,: 42524–42531.
  • Butt et al. (2003) J. Biol. Chem. 278,: 15601-15607.

Antibodies against recombinant proteins or proteins purified from natural sources:

Please consult with us before you start an expression / purification project: We may help you with some advise, for instance which fragment to choose or how to deal with insoluble antigens (inclusion bodies) without the need of elaborated solubilization procedures. Ask us for our proprietary protocol!

You provide us with your antigen of interest, we will do the rest:

 

  • Coupling to carrier proteins (if required, e.g. with incomplete or poor antigens) and coupling to affinity matrices (for affinity purification)
  • Raising antibodies either according to our standard protocol or according to your specifications
  • Affinity purification of your antibodies on one or multiple antigen cartridge(s) (even with "insoluble" antigens"!)