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Yes, there are two options:
Last update on 09.08.2011 by Matthias Reinhard.
The apparent molecular mass of LPP (and e.g. also that of the related protein Zyxin) appear to be somewhat bigger in SDS-PAGE (~76 kDa) than calculated. This is a well-known behavior of proline-rich proteins (with stretches of 3-4 and more Pro in a row).
Due to these Pro stretches the proteins bind less SDS and consequently carry less negative charge than would be expected based on their size. Less negative charge means slower migration in SDS-PAGE. This effect often is exaggerated when there is a phosphorylation near the Pro-rich region (see corresponding FAQ).
Last update on 27.12.2012 by Matthias Reinhard.
Proline-rich proteins (with stretches of 3-4 and more Pro in a row) bind less SDS than they should according to their size. Consequently they carry less negative charge and hence migrate slower in SDS-PAGE. This effect often is exaggerated when there is a phosphorylation near the Pro-rich region.
This effect is well know for several proteins, for example:
1) VASP (vasodilator-stimulated phospho protein):
2) LPP (lipoma-preferred partner):
Last update on 27.12.2012 by Matthias Reinhard.