Published work relying on immunoGlobe services (selection):

  • Wild et al. (2011) Science 333:228-33.
  • Günther et al. (2009) J. Mol. Med. 87:31-41.
  • Pagenstecher et al. (2009) Hum. Mol. Genetics 18: 911–918.
  • Fritsche et al. (2008) Nature Genetics 40: 892-896.
  • Agerer et al. (2003) J. Biol. Chem. 278,: 42524–42531.
  • Butt et al. (2003) J. Biol. Chem. 278,: 15601-15607.

Antibody Affinity Purification Schemes

Please feel free to select any affinity purification scheme that is suitable for your purposes (1 to 3 different affinity matrices; serum volume 5 ml to 50 ml or more)...

Fast affinity purification of test sera
(5 ml) and large bleeds (15-20 ml)

4
Service:
Cartridges
1 Affinity Matrix
Possible applications:
depletion of unspecific antibodies (reducing background)
Scheme:
Purification test1
Service:
Cartridges
2 Affinity Matrices
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies, (de)phosphorylation specific antibodies, cleavage site specific antibodies, antibodies against mutations, antibodies against other modifications, sub-fractionation (anti-domains, ...)
Scheme:
Purification test2
Service:
Cartridges
2 Matrices: 1 with your tagged protein + 1 shared ready-to-use tag matrix
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies
Scheme:
Purification test2 rent
Service:
Cartridges
3 Affinity Matrices
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies, (de)phosphorylation specific antibodies, cleavage site specific antibodies, antibodies against other modifications, sub-fractionation (anti-domains, ...)
Scheme:
Purification test3

Affinity purification of regular sera (50 ml)

4
Service:
Cartridges
1 Affinity Matrix
Possible applications:
depletion of unspecific antibodies (reducing background)
Scheme:
Purification regular1
Service:
Cartridges
2 Affinity Matrices
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies, (de)phosphorylation specific antibodies, cleavage site specific antibodies, antibodies against mutations, antibodies against other modifications, sub-fractionation (anti-domains, ...)
Scheme:
Purification regular2
Service:
Cartridges
2 Matrices: 1 with your tagged protein + 1 shared ready-to-use tag matrix
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies
Scheme:
Purification regular2 rent
Service:
Cartridges
3 Affinity Matrices
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies, (de)phosphorylation specific antibodies, cleavage site specific antibodies, antibodies against other modifications, sub-fractionation (anti-domains, ...)
Scheme:
Purification regular3

Large scale affinity purification