Published work relying on immunoGlobe services (selection):

  • Wild et al. (2011) Science 333:228-33.
  • Günther et al. (2009) J. Mol. Med. 87:31-41.
  • Pagenstecher et al. (2009) Hum. Mol. Genetics 18: 911–918.
  • Fritsche et al. (2008) Nature Genetics 40: 892-896.
  • Agerer et al. (2003) J. Biol. Chem. 278,: 42524–42531.
  • Butt et al. (2003) J. Biol. Chem. 278,: 15601-15607.

"… sind beide spezifisch und sehr gut, (…) bindet sogar an mittels SP6 RNA Polymerase/Retikulozytenlysat exprimiertes Protein; nach meinen Erfahrungen wird hier zwar sepzifisch aber nicht sehr viel Protein (…) synthetisiert und wirklich nur die besten kommerziellen Antikörper zeigen hier eine Bindung. Also auch hier alles wunderbar."

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Antibody Affinity Purification Schemes

 

From test to production scale | from single column to three-step fractional purifications

Please feel free to select any affinity purification scheme that is suitable for your purposes (1 to 3 different affinity matrices; serum volume 5 ml to 50 ml or more).

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Most economical anti-tag antibody removal: Shared tag-columns

Which purification for which purpose ?

Strictly speaking, not all "purified" or "affinity purified" antibodies on the market are actually affinity purified. Equivalently, not all methods of antibody purification are reasonable for all purposes. Here we explain the differences and we compare the different grades of antibody purity.

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