Article #:
0176-002
Antigen/Product:
SMN, Survival of motor neuron
Synonym(s):
SMN, BCD541, Component of gems 1, Gemin-1, SMA1 / SMA 2 / SMA3 / SMA4, SMN1 / SMN2, SMNC, SMNT, Survival motor neuron protein, T-BCD541
Uni-/SwissProt #:
Q16637, A8K0V4, Q96J51
Clone / Name:
7B10
Host Species:
mouse
Mono-/polyclonal:
monoclonal (mAB)
Isotype:
IgG1
Purity:
purified on protein A/G/...
Quantity (mass):
200 µg
Quantity (vol.):
100 µl
Price (net):
210.00 €
Cross-reactivity:
human
Applications:
WB - Western blot / immunoblot, IP - immunoprecipitation, affinity isolation / purification of native tagged protein and RNA-protein complexes
Remarks:
Control peptide ("TagIt") available as product #0179-01
Positive control (human platelet lysate) available as product #8050-50
Form:
sterile filtered liquid, with sodium azide, carrier-free
Immunization:
recombinant protein (full-length)
Epitope:
known (epitope fine mapping)
Storage:
-20°C
Shipping:
RT - ambient temperature
Availability:
in stock
Data sheet:
Figure(s):
Publications referring to this product:
1.
Kroiss, M. et al. Native purification of protein and RNA-protein complexes using a novel affinity procedure. Fly (Austin) 3, 221–228 (2009). (Article).
2.
Kroiss, M. et al. Evolution of an RNP assembly system: A minimal SMN complex facilitates formation of UsnRNPs in Drosophila melanogaster. Proc Natl Acad Sci U S A 105, 10045–10050 (2008). (Article).
3.
Petri, S., Grimmler, M., Over, S., Fischer, U. & Gruss, O. J. Dephosphorylation of survival motor neurons (SMN) by PPM1G/PP2C? governs Cajal body localization and stability of the SMN complex. J Cell Biol 179, 451–465 (2007). (Article).
4.
Otter, S. et al. A Comprehensive Interaction Map of the Human Survival of Motor Neuron (SMN) Complex. J. Biol. Chem. 282, 5825–5833 (2007). (Article).
5.
Grimmler, M. et al. Unrip, a factor implicated in cap-independent translation, associates with the cytosolic SMN complex and influences its intracellular localization. Hum. Mol. Genet. 14, 3099–3111 (2005). (Article).
6.
Grimmler, M. et al. Phosphorylation regulates the activity of the SMN complex during assembly of spliceosomal U snRNPs. EMBO Rep 6, 70–76 (2005). (Article).
7.
Narayanan, U., Achsel, T., Lührmann, R. & Matera, A. G. Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein. Mol. Cell 16, 223–234 (2004). (Article).
8.
Pillai, R. S. et al. Unique Sm core structure of U7 snRNPs: assembly by a specialized SMN complex and the role of a new component, Lsm11, in histone RNA processing. Genes Dev. 17, 2321–2333 (2003). (Article).
9.
Narayanan, U., Ospina, J. K., Frey, M. R., Hebert, M. D. & Matera, A. G. SMN, the Spinal Muscular Atrophy Protein, Forms a Pre-Import Snrnp Complex with Snurportin1 and Importin ? Hum Mol Genet 11, 1785–1795 (2002). (Article).
10.
Hebert, M. D., Shpargel, K. B., Ospina, J. K., Tucker, K. E. & Matera, A. G. Coilin methylation regulates nuclear body formation. Dev. Cell 3, 329–337 (2002). (Article).
11.
Voss, M. D. et al. Functional Cooperation of Epstein-Barr Virus Nuclear Antigen 2 and the Survival Motor Neuron Protein in Transactivation of the Viral LMP1 Promoter. J. Virol. 75, 11781–11790 (2001). (Article).
12.
Meister, G., Bühler, D., Pillai, R., Lottspeich, F. & Fischer, U. A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs. Nat. Cell Biol. 3, 945–949 (2001). (Article).
13.
Jones, K. W. et al. Direct Interaction of the Spinal Muscular Atrophy Disease Protein SMN with the Small Nucleolar RNA-associated Protein Fibrillarin. J. Biol. Chem. 276, 38645–38651 (2001). (Article).
14.
Meister, G. et al. Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. Hum. Mol. Genet. 9, 1977–1986 (2000). (Article).
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