Article #:
0092-05
Antigen/Product:
Activin Receptor IIB, extracellular domain
Clone / Name:
IG-510
Host Species:
rabbit
Mono-/polyclonal:
polyclonal (pAB) | anti-(partial sequence)
Purity:
affinity purified on the antigen
Quantity (mass):
50 µg
Quantity (vol.):
100 µl
Price (net):
249.00 € introductory offer until 2011-12-31 (regular: 295.00 €)
Cross-reactivity:
human, mouse, other not tested
Applications:
WB - Western blot / immunoblot, ELISA
Sugg. dilutions:
WB: 1:5,000 (0.1 µg/ml) detection limit below 1 ng ActR-IIB ecd; ELISA: 1:400 (1.25 µg/ml)
Remarks:
IG-510 specifically detects ActR-IIB extracellular domain (ecd) in mouse and human [lane1], not reactive with the related human proteins ActR-IIecd [lane 2], BMPR-IIecd [lane 3], BMPR-IAecd [lane 4], BMPR-IBecd [lane 5], ActR-Iecd [lane 6]. Upper panel: SDS-PAGE under non-reducing conditions, lower panel: SDS-PAGE in the presence of beta-mercapto ethanol
Assay conditions:
50 ng ecd per lane
Anti-ActR-IIB antibody 1:5,000 (100 ng/ml)
2nd AB: goat-anti-rabbit x HRP, ECL detection
IG-510 detects less than 1 ng of ActR-IIB ecd. Western blots of 0.1 to 20 ng Actr-IIB ecd per lane. Upper panel: SDS-PAGE under non-reducing conditions, lower panel: SDS-PAGE in the presence of beta-mercapto ethanol
Assay conditions:
0.1 to 20 ng ecd per lane
Anti-ActR-IIB antibody 1:5,000 (100 ng/ml)
2nd AB: goat-anti-rabbit x HRP, ECL detection
IG-510 specifically detects human ActR-IIB transfected into HEK 293 T cells. Different numbers of cells transfected either with the empty pCMV vector (control, left) or pCMV[ActR-IIB] (right) were analysed by Western blotting with IG-510 antibody 24 h after transfection.The antibody specifically detects the transfected protein and some degradation products, while there is no background in cells transfected with the empty control vector.
Assay conditions:
10,000 / 3,000 / 1,000 cells per lane
Anti-ActR-IIB antibody 1:5,000 (100 ng/ml)
2nd AB: goat-anti-rabbit x HRP, ECL detection
Form:
sterile filtered liquid, with sodium azide, stabilized with carrier, also available in carrier-free form (pls. enquire)
Immunization:
recombinant protein (partial sequence)
Epitope:
extracellular domain
Shipping:
RT - ambient temperature
Storage:
-20°C
Availability:
on stock