Available Programs:
6
Program name:
Program name:
Program name:
Program name:
Program name:
Program name:
Details of the selected program:
Program name:
PEP-14W-INCL
Number of rabbits:
2
Duration
Total duration:
14 weeks
First affinity purified test antibodies:
6 weeks after first immunisation (i.e. ~9-10 weeks after peptide order)
Program extension:
option (short term or 20 weeks and more)
Peptide(s):
Peptide selection (epitope prediction):
included
Peptide synthesis (15-mer, ≥10 mg, ≥90% pure)
up to 15-mer included; longer peptides with minor surcharge
N-terminal acetylation and/or C-terminal amidation:
included
Mass spectrum & HPLC diagram:
included
Peptide coupling (5 mg) to a carrier protein:
included
Protein antigen analysis & optimisation
Sequence check for antigenicity:
included
Sequence check for evoultionary conservation:
included
Immunization
Immunizations per rabbit:
3
Additional immunizations:
Intradermal injections at multiple sites (instead of s.c. at a single site)
included
Advanced highly efficient adjuvant:
included
Serum Collection
Pre-immune serum (5 ml):
included
Test serum (per rabbit):
5 ml
Additional test sera:
Number of regular bleeds per rabbit (~15 ml serum, each):
3 (plus exsanguination; see below)
Exsanguination (>35-45 ml serum per rabbit):
included
Affinity matrix / matrices:
Synthesis of a peptide affinity matrix (5 mg of peptide):
included
Synthesis of a peptide affinity matrix (10 mg of peptide):
option (surcharge)
Affinity Purification
Fast affinity purification of 5 ml (test serum) or 15-20 ml (large bleed) per rabbit:
included, total of two test sera (1/rabbit)
Large Scale Affinity Purifications
Affinity purification of 50 ml serum:
Ultrafiltration & dialysis against PBS or a buffer of choice:
included with optional large scale purification
Guarantee
Antibodies reactive with the antigen used for immunization:
guaranteed specific reactivity with the peptide used for immunization
Average yield of affinity purified antibodies per ml of crude serum (guideline):
50 µg/ml - 0.5 mg/ml
Support
Conceptual consulting (which sequence to express? Which tags? Which test system? ...):
included
Evaluation of test results based on submitted scans/photos:
explicitly encouraged, included
As flexible as possible:
Except for the guarantee packages, you can modify any antibody program or change it to any other program of the same category just by adding or subtracting individual modules or services (see 'OPTIONS program' and affinity purification options). This can be done upon short notice at any time during the ongoing project for any upcoming steps.