Program name:
3PEP-10W-INCL
First affinity purified test antibodies:
6.5 weeks after first immunization (i.e. ~9-10 weeks after peptide order)
Program extension:
option (short term or 20 weeks and more)
Peptide selection (epitope prediction):
included
Peptide synthesis (15-mer, ≥10 mg, ≥90% pure)
includes synthesis of 3 (!) peptides; up to 15-mers included; longer peptides with minor surcharge
N-terminal acetylation and/or C-terminal amidation:
included
Mass spectrum & HPLC diagram:
included
Peptide coupling (5 mg) to a carrier protein:
included
Protein antigen analysis & optimisation
Sequence check for antigenicity:
included
Sequence check for evoultionary conservation:
included
Immunizations per rabbit:
3
Additional immunizations:
Intradermal injections at multiple sites (instead of s.c. at a single site)
included
Advanced highly efficient adjuvant:
included
Pre-immune serum (5 ml):
included
Number of regular bleeds per rabbit (~15 ml serum, each):
2 (plus exsanguination; see below)
Additional regular bleed(s) (~15 ml serum, each)
Affinity matrix / matrices:
Synthesis of a peptide affinity matrix (5 mg of peptide):
included
Synthesis of a 2. peptide affinity matrix for pre-absorption/depletion or fractional purifications (3PEP programs)::
included
Synthesis of a 3. peptide affinity matrix for pre-absorption/depletion or fractional purifications (3PEP programs)::
included
Fast affinity purification of 5 ml (test serum) or 15-20 ml (large bleed) per rabbit:
included, fractionally purified on 3 different peptide columns; resulting in up to 6 distinct antibodies directed against 3 different peptides
Large Scale Affinity Purifications
Affinity purification of 50 ml serum:
Large Scale (50 ml serum): Depletion of unwanted ABs on additional column(s):
included with optional large scale purification
Large Scale (50 ml serum): Additional depletion (twofold) of unwanted ABs on a second column:
included with optional large scale purification
Twofold depletion of unwanted ABs on a third (!) column:
included with optional large scale purification)
Ultrafiltration & dialysis against PBS or a buffer of choice:
included with optional large scale purification
Antibodies reactive with the antigen used for immunization:
Due to possible phenomena of antigenic dominance not all 3 peptides of the antigenic mixture can be guaranteed to elicit the same strong antibody response
Conceptual consulting (which sequence to express? Which tags? Which test system? ...):
included
Evaluation of test results based on submitted scans/photos:
explicitly encouraged, included