12
Service:
Cartridges
1 Affinity Matrix
Volume:
5 ml (test serum) - 15 or 20 ml (large bleed)
Service:
Cartridges
2 Affinity Matrices
Volume:
5 ml (test serum) - 15 or 20 ml (large bleed)
Service:
Cartridges
2 Matrices: 1 with your tagged protein + 1 shared ready-to-use tag matrix
Volume:
5 ml (test serum) - 15 or 20 ml (large bleed)
Service:
Cartridges
3 Affinity Matrices
Volume:
5 ml (test serum) - 15 or 20 ml (large bleed)
Service:
Cartridges
2 Matrices: 1 with your tagged protein + 1 shared ready-to-use tag matrix
Volume:
50 ml
Service:
Cartridges
2 Matrices: 1 with your tagged protein + 1 shared ready-to-use tag matrix
Volume:
200 ml (large scale)
Details of the selected service:
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies, (de)phosphorylation specific antibodies, cleavage site specific antibodies, antibodies against other modifications, sub-fractionation (anti-domains, ...)
Scheme:
Service:
FAST-3COL
Volume:
5 ml (test serum) - 15 or 20 ml (large bleed)
Affinity matrices not included
Cartridges
3 Affinity Matrices
Buffer:
Tris-glycine buffer
Ultrafiltration:
n/a; antibody concentration ~as in original serum