First affinity purified test antibodies:
6.5 weeks after first immunization (i.e. ~9-10 weeks after peptide order)
Program extension:
option (short term or 20 weeks and more)
Peptide selection (epitope prediction):
included
Peptide synthesis (for immunization & column; ≥15 mg, ≥80% pure)
up to 15-mer included; longer peptides with minor surcharge
Synthesis of a control peptide (≥10 mg, ≥80% pure):
up to 15-mer included; longer peptides with minor surcharge
N-terminal acetylation and/or C-terminal amidation:
included
Peptide modification: phospho-amino acid (pSer, pThr, pTyr):
included; other modifications: pls. enquire
Mass spectrum & HPLC diagram:
included
Peptide coupling (5 mg) to a carrier protein:
included
Sequence check for antigenicity:
included
Sequence check for evoultionary conservation:
included
Immunizations per rabbit:
3
Additional immunizations:
Intradermal injections at multiple sites (instead of s.c. at a single site)
included
Advanced highly efficient adjuvant:
included
Pre-immune serum (5 ml):
included
Number of regular bleeds per rabbit (~15 ml serum, each):
1 (The total serum of each animal will be affinity purified on the modification and preabsorbed on the control peptide); i.e. 2 independent purifications (one per rabbit)
Additional regular bleed(s) (~15 ml serum, each)
Affinity matrix / matrices:
Synthesis of a peptide affinity matrix (5 mg of peptide):
included
Synthesis of a peptide affinity matrix (10 mg of peptide):
Synthesis of a 2. peptide affinity matrix for pre-absorption/depletion or fractional purifications (3PEP programs)::
included
Synthesis of a 3. peptide affinity matrix for pre-absorption/depletion or fractional purifications (3PEP programs)::
Affinity Purification
includes fractional affinity purification (2 columns) of 2x (~15-20 ml) serum
Depletion of unwanted ABs on a second column:
included
Large Scale (50 ml serum): Additional depletion (twofold) of unwanted ABs on a second column:
n/a
Ultrafiltration & dialysis against PBS or a buffer of choice:
n/a
Conceptual consulting (which sequence to express? Which tags? Which test system? ...):
included
Evaluation of test results based on submitted scans/photos:
explicitly encouraged, included