Program name:
PEP-10W-INCL
First affinity purified test antibodies:
6.5 weeks after first immunization (i.e. ~9-10 weeks after peptide order)
Program extension:
option (short term or 20 weeks and more)
Peptide selection (epitope prediction):
included
Epitope mapping service (for proteins up to 60 kDa, incl. micro array synthesis, fluorescence read-out, reporting)
Peptide synthesis (for immunization & column; ≥15 mg, ≥80% pure)
up to 15-mer included; longer peptides with minor surcharge
N-terminal acetylation and/or C-terminal amidation:
included
Mass spectrum & HPLC diagram:
included
Peptide coupling (5 mg) to a carrier protein:
included
Protein antigen analysis & optimisation
Sequence check for antigenicity:
included
Sequence check for evoultionary conservation:
included
Immunizations per rabbit:
3
Additional immunizations:
Intradermal injections at multiple sites (instead of s.c. at a single site)
included
Advanced highly efficient adjuvant:
included
Pre-immune serum (5 ml):
included
Test serum (per rabbit):
5 ml
Number of regular bleeds per rabbit (~15 ml serum, each):
1 (plus exsanguination; see below)
Additional regular bleed(s) (~15 ml serum, each)
Affinity matrix / matrices:
Synthesis of a peptide affinity matrix (5 mg of peptide):
included
Synthesis of a peptide affinity matrix (10 mg of peptide):
Fast affinity purification of 5 ml (test serum) or 15-20 ml (large bleed) per rabbit:
included, total of two test sera (1/rabbit)
Large Scale Affinity Purifications
Affinity purification of 50 ml serum:
Ultrafiltration & dialysis against PBS or a buffer of choice:
included with optional large scale purification
Antibodies reactive with the antigen used for immunization:
guaranteed specific reactivity with the peptide used for immunization
Average yield of affinity purified antibodies per ml of crude serum (guideline):
50 µg/ml - 0.5 mg/ml
Conceptual consulting (which sequence to express? Which tags? Which test system? ...):
included
Evaluation of test results based on submitted scans/photos:
explicitly encouraged, included