Antibody Affinity Purification Schemes
Please feel free to select any affinity purification scheme that is suitable for your purposes (1 to 3 different affinity matrices; serum volume 5 ml to 50 ml or more)...
Large scale affinity purification
(200 ml)
4
Cartridges
1 Affinity Matrix
Possible applications:
depletion of unspecific antibodies (reducing background)
Cartridges
2 Affinity Matrices
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies, (de)phosphorylation specific antibodies, cleavage site specific antibodies, antibodies against mutations, antibodies against other modifications, sub-fractionation (anti-domains, ...)
Cartridges
2 Matrices: 1 with your tagged protein + 1 shared ready-to-use tag matrix
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies
Cartridges
2 Affinity Matrices
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies, (de)phosphorylation specific antibodies, cleavage site specific antibodies, antibodies against mutations, antibodies against other modifications, sub-fractionation (anti-domains, ...)