12
Service:
Cartridges
1 Affinity Matrix
Volume:
5 ml (test serum) - 15 or 20 ml (large bleed)
Service:
Cartridges
2 Affinity Matrices
Volume:
5 ml (test serum) - 15 or 20 ml (large bleed)
Service:
Cartridges
2 Matrices: 1 with your tagged protein + 1 shared ready-to-use tag matrix
Volume:
5 ml (test serum) - 15 or 20 ml (large bleed)
Service:
Cartridges
3 Affinity Matrices
Volume:
5 ml (test serum) - 15 or 20 ml (large bleed)
Service:
Cartridges
2 Matrices: 1 with your tagged protein + 1 shared ready-to-use tag matrix
Volume:
50 ml
Service:
Cartridges
2 Matrices: 1 with your tagged protein + 1 shared ready-to-use tag matrix
Volume:
200 ml (large scale)
Details of the selected service:
Possible applications:
depletion of unspecific antibodies (reducing background), depletion of anti-tag antibodies, (de)phosphorylation specific antibodies, cleavage site specific antibodies, antibodies against other modifications, sub-fractionation (anti-domains, ...)
Scheme:
Service:
50ML-3COL
Volume:
50 ml
Comment:
Includes 3 separate cycles, each with serum recirculation, 3 washes & elution
If required (high yield / low capacity or affinity) more cycles may be added as an option
Cartridges
3 Affinity Matrices
Buffer:
PBS, any other buffer of choice
Ultrafiltration:
included; planned concentration: 0.5 to ~1.0 mg/ml (if antibody tolerates without precipitation)