Other antibodies against the same antigen:

Clone / Name:
C o l 1 - ¾ C
Host Species:
rabbit
Mono-/polyclonal:
polyclonal (pAB) | epitope selective
Purity:
affinity purified on the antigen
Quantity (mass):
50 µg
Quantity (vol.):
200 µl
Price (net):
520.00 €

 

Antibodies against related antigens:

Clone / Name:
IG-731
Host Species:
rabbit
Antigen/Product:
Clone / Name:
F1
Host Species:
rabbit
Clone / Name:
IG-731
Host Species:
rabbit
Clone / Name:
IG-817
Host Species:
rabbit
Antigen/Product:
Clone / Name:
IG-701
Host Species:
rabbit
Clone / Name:
M4
Host Species:
rabbit
Antigen/Product:
Clone / Name:
IG-706
Host Species:
rabbit
Clone / Name:
IE273
Host Species:
mouse
Article #:
0207-050
Antigen/Product:
Collagen type I, cleavage site
Synonym(s):
Col1-¾C
Also recognized:
Alpha-2 collagen type I (¾ fragment, C-terminal cleavage site, Gly775–Leu776)
Uni-/SwissProt #:
Clone / Name:
C o l 1 - ¾ C
Host Species:
rabbit
Mono-/polyclonal:
polyclonal (pAB) | epitope selective
Purity:
affinity purified on the antigen
Quantity (mass):
50 µg
Quantity (vol.):
250 µl
Price (net):
470.00 €
Cross-reactivity:
human, mouse, rat, guinea pig, dog, cat, donkey, pig, cow, sheep, chicken, others not tested
Applications:
IF - immunofluorescence
Sugg. dilutions:
IF: 2.5 µg/ml - 10 µg/ml
Fixatives tested:
formaldehyde
Remarks:

Background Information
The proteolysis of collagens plays an important role in numerous physiological and pathological situations such as morphogenesis, wound healing, arthritis, arteriosclerosis, and tumor metastasis. Triple helical type I collagens are made up of two α 1 (I) and one α 2 (I) chains, and are found in skin, tendon, ligament and interstitial tissues. Due to their fibrillary structure native collagens are resistant to most proteases. They are substrates however for certain matrix metalloproteinases (MMPs), which constitute a family of zinc-dependent enzymes catalyzing the degradation of extracellular matrix components [1,2]. Initial MMP-8 dependent cleavage of collagen into the characteristic ¾ and ¼ fragments has been shown to enable MMP-9 diffusion along the protein helix, with preferential binding to the collagen ¾ fragment tail. Finally, untwisting of the helix end results in the local denaturation of the triple helical structure [3].

  • [1] Song F., Wisithphrom K., Zhou J. & Windsor L. J. (2006). Matrix Metalloproteinase Dependent and Independent Collagen Degradation. Frontiers in Bioscience, 11:3100-20.
  • [2] Bertini I., Fragai M., Luchinat C., Melikian M., Toccafondi M., Lauer Ja. L. & Fields G. B. (2012). The Structural Basis for Matrix Metalloproteinase 1 Catalyzed Collagenolysis. J. Am. Chem. Soc. 134(4): 2100–2110.
  • [3] Rosenblum G., Van den Steen P. E., Cohen S. R., Bitler A., Brand D. D., Opdenakker G. & Sagi I. (2010). Direct Visualization of Protease Action on Collagen Triple Helical Structure. PLoS ONE 5(6): e11043.

Model depicting antibody 
detection of Col1 ¾C


Model depicting antibody detection of Col1 ¾C. MMPs cleaving the α chains, create free COOH groups at the C-terminal end of the ¾ fragment, which gets untwisted and exposes the antibody epitope. The carboxyl group proper is not part of this epitope. However, there is also a companion antibody available (IG-1246) that requires the free carboxyl group for binding (please enquire).

Form:
sterile filtered liquid, with sodium azide, stabilized with carrier
Immunization:
synthetic peptide
Epitope:
C-terminal end of the N-terminal three quarter collagen fragment (Col1 ¾), which results from MT1-MMP, MMP-1, MMP-2, or MMP-8 dependent cleavage of the α 1 (I) and α 2 (I) chains at the G775–I776 & G775–L776 bonds, respectively
Storage:
-20°C
Shipping:
RT - ambient temperature
Availability:
in stock
Data sheet:
Figure(s):
<p><strong>Collagen degradation by human breast cancer MDA-MB-231 cells embedded in a 3D collagen matrix</strong> (2.2 mg/ml). Cells have been treated with non-targeting siRNA (A) or siRNA specific for MT1-MMP (B; knock down control) for 48 hours and then transferred into collagen for 24 hours. After fixation (4% PFA at 37°C for 30 min) samples were labeled with collagen type I cleavage site antibody diluted 1:100 in PBS (2.0 μg/ml, 2 h at 4°C). Confocal photomicrograph: Anti-rabbit antibody (red), staining for F-actin (phalloidin, green), and DNA (DAPI; blue). (Data courtesy of Marie Irondelle & Dr. Philippe Chavrier)</p>
Include figures
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Include data sheet
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