Details of the selected program:

Program name:
PRO-10W-TST2-SHARED
Number of rabbits:
1
Duration
 
Total duration:
10 weeks
First affinity purified test antibodies:
after 6.5 weeks
Program extension:
option (short term or 20 weeks and more)
Protein antigen analysis & optimisation
 
Sequence check for antigenicity:
included
Sequence check for evoultionary conservation:
included
Protein coupling to a carrier protein:
Immunization
 
Immunizations per rabbit:
3
Additional immunizations:
Intradermal injections at multiple sites (instead of s.c. at a single site)
included
Advanced highly efficient adjuvant:
included
Serum Collection
 
Pre-immune serum (5 ml):
included
Test serum (per rabbit):
5 ml
Additional test sera:
Number of regular bleeds per rabbit (~15 ml serum, each):
1 (plus exsanguination; see below)
Exsanguination (>35-45 ml serum per rabbit):
included
Affinity matrix / matrices:
 
Synthesis of a protein affinity matrix (~2 ml NHS sepharose):
1 matrix included (e.g. with tagged protein of interest), includes usage of a shared second column with tag only
Affinity Purification
 
Fast affinity purification of 5 ml (test serum) or 15-20 ml (large bleed) per rabbit:
included
Depletion of unwanted ABs on a second column:
included
Support
 
Conceptual consulting (which sequence to express? Which tags? Which test system? ...):
included
Evaluation of test results based on submitted scans/photos:
explicitly encouraged, included

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As flexible as possible:

Except for the guarantee packages, you can modify any antibody program or change it to any other program of the same category just by adding or subtracting individual modules or services (see 'OPTIONS program' and affinity purification options). This can be done upon short notice at any time during the ongoing project for any upcoming steps.

Please add 5 and 4.