Available Programs:
8
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Details of the selected program:
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OPTIONS
Duration
Program extension:
15.00 € /week/rabbit; or: 20 week extension with 2 addtl. boosts and 3 addtl. large bleeds (~15 ml serum, each): 320.- €
Peptide(s):
Peptide selection (epitope prediction):
included with most peptide and all anti-modification programs
Epitope mapping service (for proteins up to 60 kDa, incl. micro array synthesis, fluorescence read-out, reporting)
675.00 €
Peptide synthesis (15-mer, ≥15 mg, ≥80% pure):
alternatative to ≥10 mg, ≥90% pure peptides; same price
Peptide synthesis (15-mer, ≥10 mg, ≥90% pure)
included with all peptide and anti-modification programs
N-terminal acetylation and/or C-terminal amidation:
included
Peptide modification: phospho-amino acid (pSer, pThr, pTyr):
included
Mass spectrum & HPLC diagram:
included
Protein antigen analysis & optimisation
Sequence check for antigenicity:
included
Sequence check for evoultionary conservation:
included
Protein coupling to a carrier protein:
175.00 €
Immunization
Additional immunizations:
29.00 €, each
Intradermal injections at multiple sites (instead of s.c. at a single site)
included
Advanced highly efficient adjuvant:
included
Serum Collection
Additional test sera:
24.00 € each (3 ml serum if no purification is required; 5 ml serum when scheduled for affinity purification)
Additional regular bleed(s) (~15 ml serum, each)
40.00 €, each
Exsanguination (>35-45 ml serum per rabbit):
included
Affinity matrix / matrices:
Synthesis of a peptide affinity matrix (5 mg of peptide):
215.00 €, peptide to be supplied; peptide & matrix included with the corresponding programs
Synthesis of a peptide affinity matrix (10 mg of peptide):
262.00 €, peptide to be supplied; alternative: 47.- € surcharge for upscaling from a 5 mg peptide matrix, which is included with the corresponding programs
Synthesis of a protein affinity matrix (~2 ml NHS sepharose):
165.00 €
Synthesis of a protein affinity matrix (~4-5 ml NHS sepharose):
225.00 €
Affinity Purification
Fast affinity purification of 5 ml (test serum) or 15-20 ml (large bleed) per rabbit:
65.00 € (1 column); 75.00 € (2 columns)
Large Scale Affinity Purifications
Affinity purification of 50 ml serum:
325 € (includes 3 independent cycles of equilibration; recirculation, 3 washes, elution); addtl. optional cycles 20.00 €, each; (see scheme)
Large Scale (50 ml serum): Depletion of unwanted ABs on additional column(s):
2 columns: 130.00 €; 3 columns: 215.00 € (surcharges; includes 3 independent cycles of equilibration; recirculation over 2 or 3 columns respectively, 3 washes, separate elution of all columns), dialysis and ultrafiltration; 1 additional depletion of the dialyzed eluate (!); addtl. optional cycles 25.00 €, each; (see 2 column scheme, 3 column scheme)
Large Scale (50 ml serum): Additional depletion (twofold) of unwanted ABs on a second column:
included with fractional large scale purification
Twofold depletion of unwanted ABs on a third (!) column:
included with fractional large scale purification (3PEP)
Ultrafiltration & dialysis against PBS or a buffer of choice:
included withl large scale purification
Support
Conceptual consulting (which sequence to express? Which tags? Which test system? ...):
included
Evaluation of test results based on submitted scans/photos:
included
As flexible as possible:
Except for the guarantee packages, you can modify any antibody program or change it to any other program of the same category just by adding or subtracting individual modules or services (see 'OPTIONS program' and affinity purification options). This can be done upon short notice at any time during the ongoing project for any upcoming steps.