Details of the selected program:

Program name:
MOD-6W-ECO
Number of rabbits:
2
Duration
 
Total duration:
6 weeks
First affinity purified test antibodies:
6.5 weeks after first immunization (i.e. ~9-10 weeks after peptide order)
Program extension:
option (short term or 20 weeks and more)
Peptide(s):
 
Peptide selection (epitope prediction):
included
Peptide synthesis (for immunization & column; ≥15 mg, ≥80% pure)
up to 15-mer included; longer peptides with minor surcharge
Synthesis of a control peptide (≥10 mg, ≥80% pure):
up to 15-mer included; longer peptides with minor surcharge
N-terminal acetylation and/or C-terminal amidation:
included
Peptide modification: phospho-amino acid (pSer, pThr, pTyr):
included; other modifications: pls. enquire
Mass spectrum & HPLC diagram:
included
Peptide coupling (5 mg) to a carrier protein:
included
Sequence check for antigenicity:
included
Sequence check for evoultionary conservation:
included
Immunization
 
Immunizations per rabbit:
3
Additional immunizations:
Intradermal injections at multiple sites (instead of s.c. at a single site)
included
Advanced highly efficient adjuvant:
included
Serum Collection
 
Pre-immune serum (5 ml):
included
Additional test sera:
Number of regular bleeds per rabbit (~15 ml serum, each):
1 (The total serum of each animal will be affinity purified on the modification and preabsorbed on the control peptide); i.e. 2 independent purifications (one per rabbit)
Additional regular bleed(s) (~15 ml serum, each)
Exsanguination (>35-45 ml serum per rabbit):
Affinity matrix / matrices:
 
Synthesis of a peptide affinity matrix (5 mg of peptide):
included
Synthesis of a peptide affinity matrix (10 mg of peptide):
Synthesis of a 2. peptide affinity matrix for pre-absorption/depletion or fractional purifications (3PEP programs)::
included
Synthesis of a 3. peptide affinity matrix for pre-absorption/depletion or fractional purifications (3PEP programs)::
Affinity Purification
includes fractional affinity purification (2 columns) of 2x (~15-20 ml) serum
Depletion of unwanted ABs on a second column:
included
Large Scale (50 ml serum): Additional depletion (twofold) of unwanted ABs on a second column:
n/a
Ultrafiltration & dialysis against PBS or a buffer of choice:
n/a
Support
 
Conceptual consulting (which sequence to express? Which tags? Which test system? ...):
included
Evaluation of test results based on submitted scans/photos:
explicitly encouraged, included

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As flexible as possible:

Except for the guarantee packages, you can modify any antibody program or change it to any other program of the same category just by adding or subtracting individual modules or services (see 'OPTIONS program' and affinity purification options). This can be done upon short notice at any time during the ongoing project for any upcoming steps.

What is the sum of 2 and 9?