Details of the selected program:

Program name:
3PEP-10W-INCL
Number of rabbits:
2
Duration
 
Total duration:
10 weeks
First affinity purified test antibodies:
6.5 weeks after first immunization (i.e. ~9-10 weeks after peptide order)
Program extension:
option (short term or 20 weeks and more)
Peptide(s):
 
Peptide selection (epitope prediction):
included
Peptide synthesis (15-mer, ≥10 mg, ≥90% pure)
includes synthesis of 3 (!) peptides; up to 15-mers included; longer peptides with minor surcharge
N-terminal acetylation and/or C-terminal amidation:
included
Mass spectrum & HPLC diagram:
included
Peptide coupling (5 mg) to a carrier protein:
included
Protein antigen analysis & optimisation
 
Sequence check for antigenicity:
included
Sequence check for evoultionary conservation:
included
Immunization
 
Immunizations per rabbit:
3
Additional immunizations:
Intradermal injections at multiple sites (instead of s.c. at a single site)
included
Advanced highly efficient adjuvant:
included
Serum Collection
 
Pre-immune serum (5 ml):
included
Additional test sera:
Number of regular bleeds per rabbit (~15 ml serum, each):
2 (plus exsanguination; see below)
Additional regular bleed(s) (~15 ml serum, each)
Exsanguination (>35-45 ml serum per rabbit):
included
Affinity matrix / matrices:
 
Synthesis of a peptide affinity matrix (5 mg of peptide):
included
Synthesis of a 2. peptide affinity matrix for pre-absorption/depletion or fractional purifications (3PEP programs)::
included
Synthesis of a 3. peptide affinity matrix for pre-absorption/depletion or fractional purifications (3PEP programs)::
included
Affinity Purification
 
Fast affinity purification of 5 ml (test serum) or 15-20 ml (large bleed) per rabbit:
included, fractionally purified on 3 different peptide columns; resulting in up to 6 distinct antibodies directed against 3 different peptides
Large Scale Affinity Purifications
 
Affinity purification of 50 ml serum:
Large Scale (50 ml serum): Depletion of unwanted ABs on additional column(s):
included with optional large scale purification
Large Scale (50 ml serum): Additional depletion (twofold) of unwanted ABs on a second column:
included with optional large scale purification
Twofold depletion of unwanted ABs on a third (!) column:
included with optional large scale purification)
Ultrafiltration & dialysis against PBS or a buffer of choice:
included with optional large scale purification
Guarantee
 
Antibodies reactive with the antigen used for immunization:
Due to possible phenomena of antigenic dominance not all 3 peptides of the antigenic mixture can be guaranteed to elicit the same strong antibody response
Support
 
Conceptual consulting (which sequence to express? Which tags? Which test system? ...):
included
Evaluation of test results based on submitted scans/photos:
explicitly encouraged, included

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As flexible as possible:

Except for the guarantee packages, you can modify any antibody program or change it to any other program of the same category just by adding or subtracting individual modules or services (see 'OPTIONS program' and affinity purification options). This can be done upon short notice at any time during the ongoing project for any upcoming steps.

What is the sum of 2 and 5?